DNA Polymerase I (E. coli) from New England Biolabs

Product Specs
ItemDNA Polymerase I (E. coli)
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0209L
Concentration10 U/μl
Quantity2500 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmoles of dNTPs to an acid-insoluble form in 30 minutes at 37°C.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Genome-wide analysis of RNA-chromatin interactions in lizards as a mean for functional lncRNA identificationBMC GenomicsAugust 7, 2023Mariela Tenorio, Joanna Serwatowska, Selene L. Fernandez-Valverde, Katarzyna Oktaba, Diego Cortezthe cDNA-linker-DNA molecules was synthesized with Escherichia coli DNA polymerase I (NEB, Cat. No. M0209L). cDNA-linker-DNA molecules were purified with magnetic beads coated with streptavidin (Dynabeads MyOne Streptavidin T1

(2) VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cellsGenome BiologyJune 23, 2023Reyna Edith Rosales-Alvarez, Jasmin Rettkowski, Josip Stefan Herman, Gabrijela Dumbović, Nina Cabezas-Wallscheid, Dominic Grün(Invitrogen; 18,021,071) and 5 × second-strand buffer were replaced with E. coli DNA polymerase (NEB, M0209L), E. coli DNA ligase (NEB, M0205L), RNaseH (NEB, M0297S), and 10 × Second-Strand Buffer (NEB, B6117S)

(3) Promoter‐pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to saltThe EMBO JournalMarch 1, 2023Miguel Montez, Maria Majchrowska, Michal Krzyszton, Grzegorz Bokota, Sebastian Sacharowski, Magdalena Wrona, Ruslan Yatusevich, Ferran Massana, Dariusz Plewczynski, Szymon SwiezewskimM,ThermoFisher,R0192 DTT,ThermoFisher,DTT001.10 E. coli DNA Ligase,New England Biolabs,M0205L E. coli DNA polymerase,New England Biolabs,M0209L EDTA Sterile Solution 0.5 M pH 8.0,BioShop,EDT111.500 Empigen,Sigma‐Aldrich,30326‐50ML Ethanol 96

(4) A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociationMolecular Systems BiologyFebruary 10, 2023Anika Neuschulz, Olga Bakina, Victor Badillo‐Lisakowski, Pedro Olivares‐Chauvet, Thomas Conrad, Michael Gotthardt, Helmut Kettenmann, Jan Philipp Junkertotal), 0.09 μl DNA ligase (New England Biolabs #M0205L), 0.35 μl E. coli DNA Pol I (New England Biolabs #M0209L), 0.09 μl RNase H (New England Biolabs #M0297L)) to the sample. If multiple samples were processed they

(5) Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNANatureJanuary 19, 2023Oleg Dmytrenko, Gina C. Neumann, Thomson Hallmark, Dylan J. Keiser, Valerie M. Crowley, Elena Vialetto, Ioannis Mougiakos, Katharina G. Wandera, Hannah Domgaard, Johannes Weber, et al.80 °C for between 1 and 30 min. The reactions were run on a 0.8% agarose gel. DNA polymerase I (NEB, M0209L) was added to the reaction (0.2 U µl−1) with the Atto421-NT labelling mix (1×) and NT labelling buffer

(6) Retrotransposon activation during Drosophila metamorphosis conditions adult antiviral responsesNature GeneticsDecember 1, 2022Lu Wang, Lauren Tracy, Weijia Su, Fu Yang, Yu Feng, Neal Silverman, Z. Z. Zhao ZhangcDNA was synthesized (RNase H, Thermo Fisher Scientific, Cat# 18021014; DNA Polymerase I, NEB, Cat# M0209L). dUTP incorporation was done in 16 °C for 2.5 hours and DNA was purified by using Ampure XP beads.

(7) Inheritance of repressed chromatin domains during S phase requires the histone chaperone NPM1Science AdvancesApril 29, 2022Thelma M. Escobar, Jia-Ray Yu, Sanxiong Liu, Kimberly Lucero, Nikita Vasilyev, Evgeny Nudler, Danny Reinbergsynthesized with deoxyuridine triphosphate to generate strand asymmetry using DNA polymerase I (NEB, M0209L) and the Escherichia coli ligase (Enzymatics, L6090L). RNA-seq libraries were constructed using the

(8) NicE-C efficiently reveals open chromatin-associated chromosome interactions at high resolutionGenome researchMarch 1, 2022Luo Z, Zhang R, Hu T, Zhu Y, Wu Y, Li W, Zhang Z, Yao X, Liang H, Song X.resuspended in the following reaction: 0.5 μL of Nt.CviPII (NEB R0626S), 2 μL of DNA Polymerase I (NEB M0209L), 3 μL of 1 mM dTTP, dGTP, biotin-14-dATP (Jena Bioscience NU-835-BIO14-S) and 1.5 μL of 1 mM biotin-14-dCTP

(9) Multilayer omics analysis reveals a non-classical retinoic acid signaling axis that regulates hematopoietic stem cell identityCell Stem CellJanuary 6, 2022Katharina Schönberger et al.ProtoScript II Reverse Transcriptase,NEB,M0368L Murine Rnase Inhibitor,NEB,M0314L "Rnase H, recombinant",NEB,M0297L DNA Polymerase I,NEB,M0209L DAPI,Sigma-Aldrich,D9542 All-trans-retinoic acid (at-RA),Sigma-Aldrich,R2625 13-cis-4oxo-Retinoic acid

(10) Baiting out a full length sequence from unmapped RNA-seq dataBMC GenomicsDecember 1, 2021Dongwei Li, Qitong Huang, Lei Huang, Jikai Wen, Jing Luo, Qing Li, Yanling Peng, Yubo ZhangcDNA and then incubated on ice for 5 min. Next, 2.5 μl of DNA polymerase I, E.coli (10 U/μl) (NEB; M0209L) and 0.5 μl of RNaseH (2 U/μl) was added to above first strand cDNA mixture and incubated at 15 °C for

(11) LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cellsScience advancesOctober 1, 2019LeRoy G, Oksuz O, Descostes N, Aoi Y, Ganai RA, Kara HO, Yu JR, Lee CH, Stafford J, Shilatifard A, et al.was synthesized with deoxyuridine triphosphate to generate strand asymmetry using DNA polymerase I [M0209L, New England Biolabs (NEB)] and the Escherichia coli Ligase (L6090L, Enzymatics). RNA-seq libraries

(12) Chromatin‐Associated RNA Sequencing (ChAR‐seq)Current Protocols in Molecular BiologyApril 1, 2019David Jukam, Charles Limouse, Owen K. Smith, Viviana I. Risca, Jason C. Bell, Aaron F. Straight(see recipe) 1 M MgCl2 (RNase-free) Escherichia coli DNA polymerase I (10 U/μl stock; NEB, cat. no. M0209L) RNase H (5 U/μl; NEB, cat. no. M0297S) 5 M NaCl (RNase-free) Proteinase K (20 mg/ml) 3 M sodium acetate,

(13) The Landscape of Circular RNA in CancerCellFebruary 7, 2019Vo JN, Cieslik M, Zhang Y, Shukla S, Xiao L, Zhang Y, Wu YM, Dhanasekaran SM, Engelke CG, Cao X, et al.30–2001™ Penicillin-Streptomycin,Invitrogen,15140122 Power SYBR Green Master Mix,Applied Biosystems,4367659 DNA Polymerase I,New England Biolabs,M0209L USER Enzyme,New England Biolabs,M5505L Critical Commercial Assays,Critical Commercial Assays,Critical

(14) A new full-length circular DNA sequencing method for viral-sized genomes reveals that RNAi transgenic plants provoke a shift in geminivirus populations in the fieldNucleic Acids ResearchJanuary 25, 2019Devang Mehta, Matthias Hirsch-Hoffmann, Mariam Were, Andrea Patrignani, Syed Shan-e-Ali Zaidi, Hassan Were, Wilhelm Gruissem, Hervé Vanderschurenwith 3 U T4 DNA polymerase (M0203L, New England Biolabs) and 10 U Escherichia coli DNA polymerase I (M0209L, New England Biolabs) with 1× NEBuffer 2 and 1mM dNTPs in a 50 μl reaction. The reaction was incubated

(15) LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma CellsImmunityJuly 17, 2018Lino AC, Dang VD, Lampropoulou V, Welle A, Joedicke J, Pohar J, Simon Q, Thalmensi J, Baures A, Flühler V, et al.,New England Biolabs,Cat. #M0202L RNase H,New England Biolabs,Cat. #M0297L DNA Polymerase I,New England Biolabs,Cat. #M0209L NEBNext High-Fidelity 2X PCR Master Mix,New England Biolabs,Cat. #M0541L TruSeq DNA PCR-Free LT Library

(16) Capturing the Onset of PRC2-Mediated Repressive Domain FormationMolecular cellJune 21, 2018Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, Blumenberg L, Karch K, Rocha PP, Garcia BA, et al.strand. Second strand was synthesized with dUTP to generate strand asymmetry using DNA Pol I (NEB, M0209L) and the E. coli ligase (Enzymatics, L6090L). RNA-seq libraries were constructed using the protocol

(17) Inactivation of CDK12 Delineates a Distinct Immunogenic Class of Advanced Prostate CancerCellJune 14, 2018Yi-Mi Yi-Mi et al.Superscript II Reverse Transcriptase,Invitrogen,18064–071 RNase H,Invitrogen,18021–071 DNA Polymerase I,New England Biolabs,M0209L USER Enzyme,New England Biolabs,M5505L Phusion High-Fidelity DNA Polymerase,New England Biolabs,M0530L Critical

(18) Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination systemPLOS ONEMarch 28, 2018Roman J. Szczesny et al.approach for generation of stable mammalian cell lines for functional studies DNA Polymerase I (NEB, M0209L), 1 U RNase H (18021–071, Thermo Fisher Scientific), and incubated at 16˚C for 2h. Further steps: purification,

(19) PA‐seq for Global Identification of RNA Polyadenylation Sites of Kaposi's Sarcoma–Associated Herpesvirus TranscriptsCurrent Protocols in MicrobiologyMay 7, 2016Ting Ni, Vladimir Majerciak, Zhi‐Ming Zheng, Jun Zhu10 mM. 10 mM dNTP mix, each (Bioline, cat. no. BIO-39029) DNA Polymerase I (E. coli) (NEB, cat. no. M0209L) RNase H (NEB, cat. no. M0297L) 10 mM Tris-HCl (pH 7.4) 100%, 80% and 70% ethanol Dynabeads MyOne Streptavidin C1

(20) RNA sequencing provides evidence for functional variability between naturally co-existing Alteromonas macleodii lineagesBMC GenomicsOctober 26, 2014Nikole E Kimes, Mario López-Pérez, Eva Ausó, Rohit Ghai, Francisco Rodriguez-Valerainstructions. The second strand was synthesized by adding 30U of E. coli Polymerase I (New England Biolabs #M0209L), 5U of E. coli DNA Ligase (New England Biolabs #M0205S), 5 U of RNase H (Epicentre #R52250), 300 μM

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